Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Intraperitoneal injection of paclitaxel produced mechanical allodynia as evidenced by the increases in paw withdrawal frequencies in response to 0.07 g ( A ) and 0.4 g ( B ) von Frey filaments on the left side. n = 12 mice/time point. * P < 0.05, ** P < 0.01 versus the vehicle-treated group at the corresponding time points by two-way ANOVA with repeated measures followed by post hoc Tukey test. ( C , D ) The levels of Nt3 mRNA ( C ) and NT3 protein ( D ) in L3/4 DRGs on different days as indicated after the first injection of paclitaxel or vehicle. D: representative Western blots (left) and a summary of the densitometric analysis (right) are shown. n = 3 biological repeats (3 mice)/time point. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( E ) The amount of NT3 protein in the L3/4 spinal cord on different days as indicated after the first injection of paclitaxel. n = 3 biological repeated (3 mice)/time point. P > 0.05 by one-way ANOVA followed by post hoc Tukey test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Injection, Produced, Western Blot

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Representative images of in situ hybridization histochemistry (ISHH) for Nt3 mRNA (red) and immunohistochemistry for β-tubulin III (green; A ) or glutamine synthetase (GS; green; B ) in the L3/4 DRGs 14 days after the first paclitaxel or vehicle injection. Cellular nuclei were labeled by 4’, 6-diamidino-2- phenylindole (DAPI; blue). n = 9–12 sections from three mice/group. * P < 0.05 by two-tailed unpaired Student t test. Scale bars: 50 µm. ( C ) Size distribution of Nt3 mRNA-labeled neuronal somata in the L3/4 DRGs 14 days after the first paclitaxel injection. Small, 25%, medium, 36%; large, 39%. ( D – H ) Representative images of ISHH for Nt3 mRNA (red) and immunohistochemistry for different DRG cell markers (calcitonin gene-related peptide (CGRP; green; D ), neurofilament 200 (NF200; green; E ), isolectin B4 (IB4; green; F ), or tyrosine hydroxylase (TH; green; G )) in the L3/4 DRGs 14 days after the first paclitaxel injection. Arrows: double-labeling neurons. Statistical summary of number of double-labeling neurons ( H ). n = 9–12 sections from three mice. Scale bar: 50 µm. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: In Situ Hybridization, Immunohistochemistry, Injection, Labeling, Two Tailed Test

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A ) Expression of NT3 protein in the ipsilateral L3/4 DRGs 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from the NT3 siRNA- or NC siRNA-microinjected male mice. n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( B – H ) Paw withdrawal frequency to 0.07 g ( B , F ) and 0.4 g ( C , G ) von Frey filament stimuli and paw withdrawal latency to heat ( D , H ) and cold ( E ) stimuli on the ipsilateral ( B – E ) and contralateral ( F – H ) side on days as indicated from male mice with DRG microinjection of NT3 siRNA or NC siRNA 7 days after the first injection of paclitaxel (PTX) or vehicle. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the paclitaxel plus NC siRNA-treated group at the corresponding time points by three-way ANOVA with repeated measures followed by post hoc Tukey test. ( I ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from male mice with DRG microinjection of NT3 siRNA or NC siRNA. n = 3 mice/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by Tukey post hoc test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Expressing, Injection, Microinjection

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A ) Expression of NT3 protein in the ipsilateral L3/4 DRGs 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from the NT3 siRNA- or NC siRNA-microinjected female mice. n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( B – H ) Paw withdrawal frequency to 0.07 g ( B , F ) and 0.4 g ( C , G ) von Frey filament stimuli and paw withdrawal latency to heat ( D , H ) and cold ( E ) stimuli on the ipsilateral ( B – E ) and contralateral ( F – H ) side on the days as indicated from female mice with DRG microinjection of NT3 siRNA or NC siRNA on day 7 after the first injection of paclitaxel or vehicle. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the paclitaxel plus NC siRNA-treated group at the corresponding time points by three-way ANOVA with repeated measures followed by post hoc Tukey test. ( I ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from female mice with DRG microinjection of NT3 siRNA or NC siRNA. n = 3 mice/group. ** P < 0.01 by two-way ANOVA followed by Tukey post hoc test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Expressing, Injection, Microinjection

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Levels of Nt3 mRNA ( A ) and NT3 protein ( B ) in the L3/4 DRGs 28 days after the first intraperitoneal (i.p.) injection of paclitaxel (PTX) or vehicle (Veh) from the tamoxifen-treated NT3 f/f mice and NT3 cKD mice. n = 3 biological repeated (3 mice)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( C – I ) Paw withdrawal frequency to 0.07 g ( C , G ) and 0.4 g ( D , H ) von Frey filament stimuli and paw withdrawal latency to heat ( E , I ) and cold ( F ) stimuli on the left ( C – F ) and right ( G – I ) side on days as indicated from NT3 f/f mice and NT3 cKD with i.p. injection of tamoxifen (Tam) daily for 7 consecutive days starting at day 7 after the first i.p. injection of paclitaxel (PTX) or vehicle. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the paclitaxel-treated NT3 f/f mice at the corresponding days by three-way ANOVA with repeated measures followed by post hoc Tukey test. ( J , K ) Spontaneous ongoing pain as assessed by the CPP test 21 days after the first i.p. injection of paclitaxel (PTX) or vehicle in the tamoxifen-treated NT3 f/f mice and NT3 cKD mice. Time spent in each chamber ( J ) and difference scores for chamber preferences calculated by subtracting preconditioning (Pre) preference time from postconditioning (Post) time spent in the lidocaine-paired chamber ( K ). n = 9 mice/group. ** P < 0.01 by three-way ANOVA with repeated measures followed by post hoc Tukey test ( J ) or by two-way ANOVA followed by Tukey post hoc test ( K ). ( L ) Levels of p-ERK1/2, total ERK1/2 and GFAP in the L3/4 dorsal horn 28 days after the first i.p. injection of paclitaxel (PTX) or vehicle in the tamoxifen-treated NT3 f/f mice and NT3 cKD mice. n = 3 mice/group. * P < 0.05, ** P < 0 . 01 by two-way ANOVA followed by post hoc Tukey test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Injection

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Levels of Nt3 mRNA ( A ) and NT3 protein ( B ) in the ipsilateral L3/4 DRGs 8 weeks after microinjection of AAV5-NT3 or control AAV5-GFP in male mice. n = 3 biological repeats (6 mice)/group. ** P < 0.01 by two-tailed unpaired Student t test. ( C – F ) Paw withdrawal frequency to 0.07 g ( C ) and 0.4 g ( D ) von Frey filament stimuli and paw withdrawal latency to heat ( E ) and cold ( F ) stimuli on the ipsilateral and contralateral sides on weeks as indicated after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the AAV5-GFP group on the ipsilateral side at the corresponding time points by three-way ANOVA with repeated measures followed by Tukey post hoc test. ( G , H ) Spontaneous ongoing pain as assessed by the CPP test 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. Time spent in each chamber ( G ) and difference scores for chamber preferences calculated by subtracting preconditioning (Pre) preference time from postconditioning (Post) time spent in the lidocaine-paired chamber ( H ). n = 9 mice/group. ** P < 0.01 by two-way ANOVA with repeated measures followed by post hoc Tukey test ( G ) or by two-tailed unpaired Student t test ( H ). ( I ) Levels of p-ERK1/2, total ERK1/2 and GFAP in the ipsilateral L3/4 dorsal horn 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 3 mice/group. ** P < 0.01 by two-tailed unpaired Student t test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Microinjection, Control, Two Tailed Test

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Levels of Nt3 mRNA ( A ) and NT3 protein ( B ) in the ipsilateral L3/4 DRGs 8 weeks after microinjection of AAV5-NT3 or control AAV5-GFP in female mice. n = 3 biological repeats (6 mice)/group. ** P < 0.01 by two-tailed unpaired Student t test. ( C – F ) Paw withdrawal frequency to 0.07 g ( C ) and 0.4 g ( D ) von Frey filament stimuli and paw withdrawal latency to heat ( E ) and cold ( F ) stimuli on the ipsilateral and contralateral sides on weeks as indicated after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the AAV5-GFP group on the ipsilateral side at the corresponding time points by three-way ANOVA with repeated measures followed by the Tukey post hoc test. ( G , H ) Spontaneous ongoing pain as assessed by the CPP test 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. Time spent in each chamber ( G ) and difference scores for chamber preferences calculated by subtracting preconditioning (Pre) preference time from postconditioning (Post) time spent in the lidocaine-paired chamber ( H ). n = 9 mice/group. ** P < 0.01 by two-way ANOVA with repeated measures followed by post hoc Tukey test ( G ) or by two-tailed unpaired Student t test ( H ). ( I ) Levels of p-ERK1/2, total ERK1/2 and GFAP in the ipsilateral L3/4 dorsal horn 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 3 mice/group. ** P < 0.01 by two-tailed unpaired Student t test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Microinjection, Control, Two Tailed Test

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Levels of Nt3 , TrkC , and p75 NTR mRNAs ( A ) and their respective proteins ( B ) in the ipsilateral L3/4 DRGs 42 days after microinjection of AAV5-NT3 (NT3) or AAV5-GFP (GFP) into unilateral L3/4 DRGs of the mice with co-microinjection of control scrambled siRNA (Scr-si), TrkC siRNA (TrkC-si), or p75 NTR siRNA (p75-si). n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. ( C – I ) Paw withdrawal frequencies to 0.07 g ( C , G ) and 0.4 g ( D , H ) von Frey filament stimuli and paw withdrawal latencies to heat ( E, I ) and cold stimuli ( F ) on the ipsilateral ( C – F ) and contralateral ( G – I ) sides on days as indicated after AAV-NT3 or AAV-GFP microinjection in the mice with co-microinjection of control scrambled siRNA (Scr-si), TrkC siRNA (TrkC-si), or p75 NTR siRNA (p75-si) starting at 35 days after AAV5 microinjection. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the AAV5-NT3 plus control scrambled siRNA-treated group at the corresponding days by two-way ANOVA with repeated measures followed by Tukey post hoc test. ( J ) Levels of p-ERK1/2, total ERK1/2 and GFAP in the ipsilateral L3/4 dorsal horn on day 42 after microinjection of AAV5-NT3 (NT3) or AAV5-GFP (GFP) into unilateral L3/4 DRGs of the mice with co-microinjection of control scrambled siRNA (Scr-si), TrkC siRNA (TrkC-si), or p75 NTR siRNA (p75-si). n = 3 mice/group. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by Tukey post hoc test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Microinjection, Control

Journal:

Article Title:

doi:

Figure Lengend Snippet: Data: mean ± SD. Data: mean ± SD. ( A – C ) Levels of CCL2 and TrkC proteins in the ipsilateral L3/4 DRGs 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from the NT3 siRNA- or negative control scrambled siRNA (NC siRNA)-microinjected male ( A ) and female ( B ) mice or 28 days after the first injection of paclitaxel or vehicle from the tamoxifen-treated NT3 f/f mice and NT3 cKD mice ( C ). n = 3 biological repeats (6 mice in A and B and 3 mice in C)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( D ) Level of CCL2 protein in the ipsilateral L3/4 DRGs 42 days after microinjection of AAV5-NT3 (NT3) or AAV5-GFP (GFP) into unilateral L3/4 DRGs of the mice with co-microinjection of control scrambled siRNA (Scr-si), or TrkC siRNA (TrkC-si). n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. ( E – H ) Levels of Nt3 mRNA ( E , G ), Ccl2 mRNA ( E , G ) and their respective proteins ( F , H ) in the cultured DRG neurons (E-F) or in human neuroblastoma cell lines ( G , H ) with transfection of NT3 siRNA or negative control scrambled siRNA (NC siRNA) plus with exposure to paclitaxel (PTX) or vehicle (Veh). n = 3 biological repeats. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. ( I , J ) Levels of Nt3 mRNA ( I ), Ccl2 mRNA ( I ) and their respective proteins ( J ) in the cultured DRG neurons with transduction of AAV5-NT3 (NT3) or AAV5-GFP (GFP) plus with transfection of NT3 siRNA or negative control scrambled siRNA (NC siRNA). n = 3 biological repeats. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Injection, Negative Control, Microinjection, Control, Cell Culture, Transfection, Transduction

Journal:

Article Title:

doi:

Figure Lengend Snippet: Co-expression analysis of Nt3 mRNA, TrkC mRNA and Ccl2 mRNA in individual large (>35 μm in diameter; A ), medium (25–35 µm in diameter; B ), and small (<25 μm in diameter; C ) DRG neurons from naive mice ( n = 2) by single-cell RT-PCR assay. NeuN mRNA is used as a neuronal marker. Tuba-1α mRNA is used as a loading control. M DNA ladder marker, NC no cDNA. n = 5 neurons/size. .

Article Snippet: The primary antibodies included rabbit anti-NT3 (1:250; Alomone Labs, catalog number: ANT-003; Jerusalem, Isreael), rabbit anti-MCP-1 (1:1000, catalog number: ab315478; Abcam; Waltham, MA), rabbit anti-p75NTR (1:1000, catalog number: 07-476; Sigma-Aldrich), rabbit anti-TrkC (1:400, catalog number: ANT-020; Alomone), rabbit anti-ERK1/2 (1:1000, catalog number: 4370S; Cell Signaling Technology), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2; 1:1000; catalog number: 3670S; Cell Signaling Technology), mouse anti-GFAP (1:1000, catalog number: 3670S; Cell Signaling Technology), rabbit anti-GAPDH (1:1000, catalog number: G9545; Sigma-Aldrich) and mouse anti-actin (1:1000, catalog number: sc-47778; Santa Cruz).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Panax notoginsenoside Rb1 ameliorates Alzheimer’s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

doi: 10.3892/etm.2013.1215

Figure Lengend Snippet: qPCR analysis of the relative levels of brain-derived neurotrophic factor (BDNF) and Tau mRNA, modulated by Panax notoginsenoside Rb1 (PNRb1). Values of the blank control group were taken as one unity to calculate the fold increase. mRNA levels were normalized by glyceraldehyde 3-phosphate dehydrogenase mRNA, whose level did not change during culture with PNRb1. Results are the means of at least three experiments. Values are the mean ± SE.

Article Snippet: Subsequently, the blots were blocked with normal goat serum antibody, incubated in rabbit anti-rat phosphorylated Tau protein and BDNF polyclonal antibody (1:1,000 and 1:600, respectively; Boster, Wuhan, China) at 4°C overnight, then washed in phosphate-buffered saline with 0.1% Triton X-100, three times for 15 min each.

Techniques: Derivative Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Panax notoginsenoside Rb1 ameliorates Alzheimer’s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

doi: 10.3892/etm.2013.1215

Figure Lengend Snippet: Immunoblot analysis of the relative expression levels of brain-derived neurotrophic factor (BDNF) and phosphorylated Tau protein, modulated by Panax notoginsenoside Rb1 (PNRb1). Values of untreated astrocytes (blank control group) were taken as one unity to calculate the fold increase. Protein levels were normalized by β-tubulin, whose level did not change during culture with PNRb1. Results are the means of at least three experiments. Values are the mean ± SE.

Article Snippet: Subsequently, the blots were blocked with normal goat serum antibody, incubated in rabbit anti-rat phosphorylated Tau protein and BDNF polyclonal antibody (1:1,000 and 1:600, respectively; Boster, Wuhan, China) at 4°C overnight, then washed in phosphate-buffered saline with 0.1% Triton X-100, three times for 15 min each.

Techniques: Western Blot, Expressing, Derivative Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Panax notoginsenoside Rb1 ameliorates Alzheimer’s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

doi: 10.3892/etm.2013.1215

Figure Lengend Snippet: Correlation between the relative expression of (A) brain-derived neurotrophic factor (BDNF) or (B) phosphorylated Tau protein and Panax notoginsenoside (PNRb1) concentration. The AD tissues were treated with 240 μm PNRb1 for 4 h. Statistical analysis was performed using the Spearman’s rank correlation test. Values of rho between 1 and 0.5 indicate a strong positive correlation, while values between −1 and −0.5 imply a strong negative correlation.

Article Snippet: Subsequently, the blots were blocked with normal goat serum antibody, incubated in rabbit anti-rat phosphorylated Tau protein and BDNF polyclonal antibody (1:1,000 and 1:600, respectively; Boster, Wuhan, China) at 4°C overnight, then washed in phosphate-buffered saline with 0.1% Triton X-100, three times for 15 min each.

Techniques: Expressing, Derivative Assay, Concentration Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Improving Control of Gene Therapy-Based Neurotrophin Delivery for Inner Ear Applications

doi: 10.3389/fbioe.2022.892969

Figure Lengend Snippet: List of primary antibodies used for immunohistochemistry.

Article Snippet: Anti-TrkB , Alomone Labs , ANT-019-AG , 1 , 1:50 (20) , Rabbit , Polyclonal.

Techniques: Immunohistochemistry, Concentration Assay